Abstract

Alzheimer’s Disease (AD) is a neurological disorder caused by acetylcholinesterase (AChE) via termination of the action of acetylcholine by catalytic hydrolysis. Inhibition of AChE is considered a useful approach to combat AD. The aim of the study is to evaluate the AChE inhibitory activity of compound isolated from the stem of Tinospora cordifolia. Chromatographic techniques were used to isolate and purify bioactive compounds. Their structures were determined by spectroscopic analysis. Ellman colorimetric assay method was used to determine the AChE inhibitory activity In vitro. The selected PDB was modeled with MOE 2019 using PDB ID: 4EY7. Chromatographic separation yielded one compound. Based on spectroscopic data, the molecule was identified as rel-(2S, 3S, 4R, 1 6E)- 2-[(2’R)-2’-hydroxynonadecanoylamino]-heneicosadec-16-ene-1,3,4-triol reported for the first time in Menispermaceae. The molecule demonstrated good AChE inhibitory activity (IC50 = 0.055±0.00 mg/mL) at 0.1 mg/mL compared to eserine (IC50 = 0.009±0.00 mg/mL). The docked pose had an abundance of hydrophobic, hydrogen and Pi stacking interaction. The ceramide form potential lead for new AChEi drug for the management of AD.

Keywords: Tinospora cordifolia, Menispermaceae, Ceramide, Acetylcholinesterase inhibition, Molecular docking